WebApr 27, 2001 · Analysis of tetraspanin/CD9P-1 interactions by Western blotting. A, unlabeled HeLa cells were lysed in Brij 97 or digitonin, and the solubilized proteins were immunoprecipitated (IP) as indicated at the top of each lane before Western blot analysis with biotin-labeled anti-CD9P-1 mAb 1F11 or anti-tetraspanin mAbs. Low amounts of … WebApr 6, 2024 · Tetraspanin CD81 plays major roles in cell-cell interactions and the regulation of cellular trafficking. This cholesterol-embarking transmembrane protein is a co-receptor for several viruses, including HCV, HIV-1 and Chikungunya virus, which exploits the large extracellular loop EC2 for cell entry. CD81 is also an anticancer target implicated in …
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WebJan 6, 2011 · CD9P-1 expression correlates with the metastatic status of lung cancer, and a truncated form of CD9P-1, GS-168AT2, inhibits in vivo tumour growth. by W Guilmain, S … Webproteins are most likely CD9P-1, EWI-2, and the integrin 3 1 which associate with tetraspanins in all cells studied so far ( Fig. 1 A ; Charrin et al., 2001 , 2003a ; Stipp et al., 2001 ). As a negative control, the integrin 5 1 did not immunoprecipitate CD9 or CD81 (no band comigrating with CD9 or CD81 was de- WebFeb 15, 2006 · Two major molecular partners of CD9 and CD81 have recently been identified, CD9P-1 and EWI-2. As seen in Fig. 3, the anti-CD9P-1 mAb 8G1 stained the oocyte, indicating expression of CD9P-1. CD9P-1 expression was also observed on oocytes in the ovary (data not shown). Sperm failing to fuse with zona-intact CD81 −/− eggs … dynamic cards inc