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WebApr 11, 2024 · After diet-induction, mice were fasted for 14 h and sacrificed, and for refeeding studies, mice were fasted and continuously refed for 2 h, 4 h, 8 h and 24 h and then were sacrificed. Additional details of methods are listed in Supplementary information. Hepatic IDH2-deficiency can resist diet-induced obesity and improve insulin sensitivity WebFeb 28, 2024 · Gene expression in the livers of fasted and refed WT and hepatocyte-Srebf-2-/-mice. Total RNA from livers of each mouse liver described in Table 2 was subjected to real-time RT-PCR as described in ‘Materials and methods.’ ApoB was used as the invariant control mRNA. Each value represents the amount of mRNA relative to that in fasted WT … bishop ford catholic high school brooklyn ny
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WebFeb 2, 2024 · This fasted-to-refed transition is closely related to the expression of Fgf21. Further in vitro and in vivo studies showed that fasting-sensitive PPARα is indispensable for the expression of Fgf21 and its protective effect on NAFLD. These findings indicated that long-term ADF protects mouse livers against HFD induced fatty liver disease ... WebDetails of the study groups are listed in Table 1. The fed, fast and refed groups had three replicates each. mRNA–miRNA expression density plots in the fed, fasted and refed … WebMay 1, 2005 · The fasted group was fed standard diet ad libitum for 24 h then fasted for 24 h. The refed group had free access to food for 8 h after injection. They were then fasted for 24 h then refed a high-carbohydrate/low-fat (HC) diet (75% wt/wt sucrose, 15% wt/wt protein, and 3% wt/wt fat) for 16 h. Mice were killed 48 h after Ad injection. bishop ford central catholic high school ny