Gh117a
WebNov 5, 2024 · To identify an efficient exolytic β-agarase required for NA2 production from agarose, the GH50A β-agarase gene from agar-degrading Cellvibrio sp. KY-GH-1 was overexpressed as a recombinant His-tagged protein using the … WebTime-kinetic analysis of GH117A α-NABH-catalyzed hydrolysate of NA2 by TLC and LC-MS/MS. a NA2 (0.4%) was incubated with purified GH117A α-NABH (10 μg/mL) in 50 mM Tris-HCl (pH 7.5) at 35 °C ...
Gh117a
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WebMay 31, 2024 · The presence of GH117A α-NABH homologues in all of the agar-degrading bacteria aligned suggests that GH117A α-NABH hydrolyzing NA2 into L-AHG and D … WebMay 18, 2024 · Results indicate that the recombinant GH117A α-NABH is NA2-specific and useful to produce L-AHG from NA2, an essential component of the agar-degrading enzyme machinery. View 3 excerpts, cites background and methods Metatranscriptomic analysis of functional response of colonic microbiota to different dietary fibers in growing pigs
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WebTwo GH117 family α-neoagarobiose hydrolases (GH117A α-NABH and GH117B α-NABH) from the freshwater agar-degrading Cellvibrio sp. KY-GH-1 were expressed and purified as recombinant His-tagged... WebGH50A β-agarase that consists of 797 amino acids was able to produce predominantly NA2 from agarose at an optimal temperature and pH of 35°C and 7.5, respectively. The enzyme was stable up to 35°C...
WebMar 30, 2024 · In this review, we describe the benefits of recombinant GH50A β-agarase and GH117A α-NABH originated from Cellvibrio sp. KY-GH-1, which may be useful for …
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